Tripterygium Wilfordii Extract Triptolide

Tripterygium wilfordii is an endangered plant of the genus Tripterygium, which belongs to the family Celastraceae. In “Supplements for the Body,” it is stated to “open the duodenum and heal pain in the joints and bones.” Phytochemical analysis revealed that the dried leaves contained more than 100 compounds, including diterpenes, triterpenes, and alkaloids. One of the best characterized compounds was triptolide (molecular formula C20H24O6, CAS 38748-32-2).

Structurally, Tripterygium Wilfordii Extract Triptolide is a rosinan-type epoxy diterpene lactone consisting of three adjacent epoxy groups and an α,β-unsaturated five-membered lactone ring. These high-energy sections contribute to higher performance and power. The purity of the commercial product was ≥98% and appeared as a crystalline powder. They are soluble in DMSO and ethanol but are insoluble in water and should be stored at −20°C away from light.

• Rheumatoid arthritis (RA): A randomized controlled trial of more than 1,000 RA patients at 20 hospitals in China showed that oral triptolide (with a response rate of 0.2 to 0.3 ACR/tab) over 12 weeks reached 26%. The addition of methotrexate significantly reduced CRP and ESR.

• Ulcerative colitis (UC): A new mouse model from Capital Medical University showed that administration of triptolide using dexamethasone-exosome encapsulation improved colonic histopathology and reduced inflammation in the colon.

• CAR-T therapy-related cytokine release syndrome (CRS): The first model from Jiangsu Provincial Chinese Medical Hospital showed that intravenous injection of triptolide could reduce hyperactivated monocytes as hypertension, hypertension as hypertension, IL6 hypertension as hypertension and IL6.

Tripterygium Wilfordii Extract Triptolide contains 0.001%-0.008% (w/w) of triptolides, which means that 10–80 g of dry leaf extract can be selected per tonne. The procedure used 70% ethanol reflux extraction followed by macroporous resin formation followed by silica gel column chromatography followed by crystallization. This procedure can be scaled up to 100-gram volumes, where HPLC purity is ≥98%.

The structure of multiple
• Transcription Factor Response: TFIIH binds to the XPB subunit and inhibits RNA polymerase II-mediated transcription elongation, thereby inhibiting the production of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6).
• Signaling responses: inhibition of IKKβ/NF-κB and JAK2/STAT3 and decreased expression of various chemokines; It simultaneously activates MAPK-p38 and caspase cascades, leading to tumor cell apoptosis.
• Epigenetic Response: downregulation of DNA methyltransferase DNMT1, upregulation of p16 and p21 gene expression, and increased sensitivity to chemotherapy.

At the cellular level
• Inhibition of DC (DC) maturation and antigen presentation, reducing T-cell activation;
• induce apoptosis in activated monocytes, rescue resting immune cells, and provide a “direct fighter” for the treatment of CRC;
• Inhibition of vascular endothelial growth factor (VEGF) secretion in tumor tissues and inhibition of angiogenesis.